Effects of Carbohydrate Mouthwash on 40 Km Cycling Performance in Triathlete
Abstract
The aim of this study was to investigate the effects of carbohydrate mouth rinse on triathletes’ performance in a 40 km cycling test, following 1.5km swim. In the randomized, blinded, and crossover experimental design, thirteen male triathletes (age: 29.69 ± 7.8 years; training experience: 2.5 ± 1.19 years; O2max: 51.33 ± 6.04 ml.kg-1.min-1) performed a time trial with self-regulated speed in two conditions: placebo mouth rinse (PLA) and carbohydrate mouth rinse (CHO). The results showed no significant differences in the mean durations of the time trial (PLA: 72min13s ± 3min30s and CHO: 72min55s ± 3min20s; p = 0.789; d = 0.08), relative power output (PLA: 2.74 ± 0.23w/kg; CHO: 2.74 ± 0.25w/kg; p = 0.945; d = 0.02), mean speed (PLA: 33.04 ± 1.28km/h; CHO: 32.98 ± 1.21km/h; p = 0.785; d = 0.09), mean Heart Rate (PLA: 148.00 ± 11.51bpm; CHO: 149.00 ± 10.78bpm), and Rate of Perceived Exertion (PLA: 5.41 ± 0.99; CHO: 5.50 ± 1.22; p = 0.704; d = 0.08). In conclusion, carbohydrate mouth rinse may not be effective to improve performance in triathletes.
References
Ambrosini, L., Presta, V., Galli, D., Mirandola, P., Vitale, M., Gobbi, G., & Condello, G. (2022). Interlink between physiological and biomechanical changes in the swim-to-cycle transition in triathlon events: A narrative review. Sports Medicine - Open, 8, 129. https://doi.org/10.1186/s40798-022-00521-z
Balke, B., & Ware R.W. (1959). An experimental study of physical fitness of Air Force personnel. Armed Forces Medical Journal, 10(6), 675-688. https://pubmed.ncbi.nlm.nih.gov/13659732/
Brietzke, C., Franco-Alvarenga P.E., Coelho-Júnior, H.J., Silveira, R., Asano, R.Y., & Pires, F.O. (2019). Effects of Carbohydrate Mouth Rinse on Cycling Time Trial Performance: A Systematic Review and Meta-Analysis. Sports Medicine, 49(1), 57-66. https://doi.org/10.1007/s40279-018-1029-7
Carter, J.M., Jeukendrup, A.E., & Jones, D.A. (2004). The effect of carbohydrate mouth rinse on 1-h cycle time trial performance. Medicine & Science in Sports & Exercise, 36, 2107-2111. https://doi.org/10.1249/01.mss.0000147585.65709.6f
Chamari, K., Haddad, M., Wong, D.P., & Dellal, A. (2012). Injury rates in professional soccer players during Ramadan. Journal of Sports Sciences, 30, 93-102. https://doi.org/10.1080/02640414.2012.696674
Chambers, E.S., Bridge, M.W., & Jones, D.A. (2009). Carbohydrate sensing in the human mouth: effects on exercise performance and brain activity. The Journal of Physiology, 587(8), 1779-1794. https://doi.org/10.1113/jphysiol.2008.164285
Chryssanthopoulos, C., Ziaras, C., Oosthuyse, T., Lambropoulos, I., Paradisis, G., Zacharogiannis, E., Philippou, A., & Maridaki, M. (2018). Carbohydrate mouth rinse does not affect performance during a 60-min running race in women. Journal of Sports Sciences, 36(7), 824-833. https://doi.org/10.1080/02640414.2017.1344358
Clarke, N.D., James, T.R., Marion, K., Roger, R., & Anne, D. (2017). No dose-response effect of carbohydrate mouth rinse concentration on 5-km running performance in recreational athletes. Journal of Strength and Conditioning Research, 31(3), 715-720. https://doi.org/10.1519/JSC.0000000000001531
Cohen J. (1998). Statistical Power Analysis for the Behavioral Sciences (2nd ed.). Hillsdale, NJ: Lawrence Erlbaum Associates, Publishers.
Deng, H., Fan, X., Liu, P., Song, T., Ahmad Fuaad, AAH, Mohd Nasiruddin, NJB, & Naharudin, MNB (2025). Fed, not fasted: Is carbohydrate mouth rinsing still ergogenic? A three-level meta-analysis. Journal of the International Society of Sports Nutrition, 22(1), 2579027. https://doi.org/10.1080/15502783.2025.2579027
Devenney, S., Collins, K. , & Shortall, M. (2016). Effects of various concentrations of carbohydrate mouth rinse on cycling performance in a fed state. European Journal of Sport Science, 16(8), 1073-1078. https://doi.org/10.1080/17461391.2016.1196735
Duric, S., Leite, LB, Forte, P., Andrade, MS, Cuk, I., Nikolaidis, PT, Weiss, K., Rosemann, T., & Knechtle, B. (2025). Physiological and biomechanical aspects of the first female finisher in the longest triathlon in the world: Triple Deca in Ultra Triathlon Italy 2024. EXCLI Journal, 24, 1461–1477. https://doi.org/10.17179/excli2025-8612
Ferreira, AMJ, Farias-Junior, LF, Mota, TAA, Elsangedy, HM, Marcadenti, A., Lemos, TMAM, Okano, AH, & Fayh, APT (2018). Carbohydrate mouth rinse and hydration strategies on cycling performance in 30 Km time trial: a randomized, crossover, controlled trial. Journal of Sports Science and Medicine, 17(2), 181-187. https://pubmed.ncbi.nlm.nih.gov/29769818/
García-Giménez, A. (2024). Triathlon performance: Physiological and training strategies from super sprint to long distance events. Physical Education and Sport Through the Centuries, 11(2), 87–93. https://doi.org/10.5937/spes2402087G
Grivas, G.V., & Alkasasbeh, W.J. (2026). Energy availability as a neurocognitive regulator of endurance performance: Integrating metabolic, perceptual, and decision-making mechanisms—A narrative review. Sports, 14(4), 150. https://doi.org/10.3390/sports14040150
Hartley, C., Carr, A., Roberts, S.S.H., Bredie, W.L.P., & Keast, R.S.J. (2024). Carbohydrate oral rinsing, cycling performance and individual complex carbohydrate taste sensitivity. Nutrients, 16(3), 459. https://doi.org/10.3390/nu16030459
Hooper, S.L., & MacKinnon, L.T. (1995). Monitoring overtraining in athletes. Recommendations. Sports Medicine, 20(5), 321-327. https://doi.org/10.2165/00007256-199520050-00003
Jackson, A.S., & Pollock, M.L. (1978). Generalized equations for predicting body density of men. British Journal of Nutrition, 40(3), 497-504. https://doi.org/10.1079/bjn19780152
Jeffers, R., Shave, R., Ross, E., Stevenson, E., & Goodall, S. (2015). The effect of carbohydrate mouth-rinse on neuromuscular fatigue following cycling exercise. Applied Physiology, Nutrition, and Metabolism, 40(6), 557-564. https://doi.org/10.1139/apnm-2014-0393
Jeukendrup, A.E., Jentjens, G.L.P., & Moseley, L. (2005). Nutritional Considerations in Triathlon. Sports Medicine, 35(2), 163-181. https://doi.org/10.2165/00007256-200535020-00005
Jeukendrup, A.E. (2011). Nutrition for endurance sports: marathon, triathlon, and road cycling. Journal of Sports Sciences, 29(1), S91-S99. https://doi.org/10.1080/02640414.2011.610348
Karayigit, R., Forbes, S.C., Naderi, A., Candow, DG, Yildirim, UC, Akca, F., Aras, D., Yasli, BC, Sisman, A., Mor, A., & Kaviani, M. (2021). Different doses of carbohydrate mouth rinse have no effect on exercise performance in resistance trained women. International Journal of Environmental Research and Public Health, 18, 3463. https://doi.org/10.3390/ijerph18073463
Korohod, T., Pietrzak-Chmiel, M., Ramaneckaitė, P., Binkiewicz, A., Ślesik, M., Tadulewicz, I., Łatkowska, S., Pawlik, A., & Pudzianowska, A. (2026). Carbohydrate mouth rinsing in recreational athletes: A narrative review of performance effects, mechanisms, and gastrointestinal implications. Quality in Sport, 51, 68660. https://doi.org/10.12775/QS.2026.51.68660
Lopes, R.F., Osiecki, R., & Rama, L. (2012). Resposta da frequência cardíaca e da concentração de lactato após cada segmento do Triathlon olímpico. Revista Brasileira de Medicina do Esporte, 18(3). https://doi.org/10.1590/S1517-86922012000300003
Miguel-Ortega, Á., Rodríguez-Rodrigo, M.-A., Mielgo-Ayuso, J., & Calleja-González, J. (2025). Triathlon: Ergo nutrition for training, competing, and recovering. Nutrients, 17(11), 1846. https://doi.org/10.3390/nu17111846
Morton, JP, Fell, JM, Gonzalez, JT, Hearris, MA, Podlogar, T., Pugh, JN, & Wallis, GA (2026). From metabolism to medals: Contemporary perspectives and revisiting carbohydrate guidelines for fueling endurance athletes during exercise. The Journal of Nutrition, 156(5), 101442. https://doi.org/10.1016/j.tjnut.2026.101442
Murray, K., Paris, L.H., Fly, A.D., Chapman, R.F., & Mickleborough, T.D. (2018). Carbohydrate mouth rinse improves cycling time-trial performance without altering plasma insulin concentration. Journal of Sports Science and Medicine, 17(1), 145-152. https://pmc.ncbi.nlm.nih.gov/articles/PMC5844201/
Pacheco, A.G., Leite, G.S., Lucas, R.D.D., & Guglielmo, L.G. (2012). A influência da natação no desempenho do triathlon: implicações para o treinamento e competição. Revista Brasileira de Cineantropometria e Desempenho Humano, 14(2), 125-241. https://doi.org/10.5007/1980-0037.2012v14n2p232
Painelli, V.S., Brietzke, C., Franco-Alvarenga, P.E., Canestri, R., Vinícius, Í., & Pires, F.O. (2022). A narrative review of current concerns and future perspectives of the carbohydrate mouth rinse effects on exercise performance. SAGE Open Medicine, 10, 20503121221098120. https://doi.org/10.1177/20503121221098120
Pires, F., Brietzke C., Pinheiro, F., Katherine, V., Mattos, E., Rodacki, A., & Ugrinowitsch, C. (2018). Carbohydrate mouth rinse fails to improve four-kilometer Cycling time trial performance. Nutrients, 10(3), 342. https://doi.org/10.3390/nu10030342
Sareban, Z., Zugel, D., Koehler, K., Hartveg, P., Zugel, M., Schumann, U., Steinacker, JM, & Treff, G. (2016). Carbohydrate intake in form of gel is associated with increased gastrointestinal distress but not with performance differences compared with liquid carbohydrate ingestion during simulated long-distance triathlon. International Journal of Sport Nutrition and Exercise Metabolism, 26(2), 114-122. https://doi.org/10.1123/ijsnem.2015-0060
Silva, R.A.D. da, Szmuchrowski, L.A., Simonini, P., Flôr, G.A.G., Silva, B.V.C. da, & Drummond, M.D.M. (2020). Effect of 12-hour fasting promoted by breakfast omission on acute weight loss and physical performance of taekwondo athletes. Archives of Budo Science of Martial Arts and Extreme Sports, 16(1), 29. https://smaes.archbudo.com/view/abstract/id/13855
Silva, T.A., Souza, M.E.C.A., Amorim, J.F., Stathis, C.G., Leandro, C.G., & Lima-Silva, A.E. (2014). Can Carbohydrate Mouth Rinse Improve Performance during Exercise? A Systematic Review. Nutrients, 6, 1-10. https://doi.org/10.3390/nu6010001
World Triathlon. 2023 Competition Rules. https://www.triathlon.org/uploads/docs/World-Triathlon_Competition-Rules_2023_20230208.pdf
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